Plasmid_Backbone

Part:BBa_J428368:Design

Designed by: Marcos Valenzuela-Ortega   Group: 2021 Engineering Committee   (2022-06-15)


pJUMP44-2A SpecR Type IIS Level 2 vector. Origin pRO1600/ColE1


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found at 3575
    Illegal suffix found at 16
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 3575
    Illegal SpeI site found at 17
    Illegal PstI site found at 31
    Illegal NotI site found at 24
    Illegal NotI site found at 3581
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 3575
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found at 3575
    Illegal suffix found at 17
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found at 3575
    Plasmid lacks a suffix.
    Illegal XbaI site found at 3590
    Illegal SpeI site found at 17
    Illegal PstI site found at 31
    Illegal NgoMIV site found at 1039
    Illegal NgoMIV site found at 1441
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal BsaI site found at 3597
    Illegal BsaI.rc site found at 10


Design Notes

Marcos Valenzuela-Ortega, Christopher French, Joint universal modular plasmids (JUMP): a flexible vector platform for synthetic biology, Synthetic Biology, Volume 6, Issue 1, 2021, ysab003, https://doi.org/10.1093/synbio/ysab003


Source

Marcos Valenzuela-Ortega, Christopher French, Joint universal modular plasmids (JUMP): a flexible vector platform for synthetic biology, Synthetic Biology, Volume 6, Issue 1, 2021, ysab003, https://doi.org/10.1093/synbio/ysab003

References